Imaging & Flow Cytometry Core
Address: L6-11, 6/F, Laboratory Block, 21 Sassoon Road, Pokfulam Hong Kong

Tel: 39177557
E-mail: corefac@hku.hk

Equipment — Flow Cytometry

BD FACSAria Fusion Cell Sorter:

BD Aria Fusion - Basic Operation Procotol
Staff-in-charge:Dr. Liu, Jing (Emily)

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BD FACSMelody Cell Sorter:

  • 488nm blue laser:
    1. FITC (527/32nm)
    2. PE (586/42nm)
    3. PerCP-Cy5.5(700/54nm)
    4. PE-Cy7 (783/56nm)
  • 633nm red laser:
    1. APC (660/10nm)
    2. APC-Cy7(783/56nm)
  • 407nm violet laser:
    1. DAPI / Pacific Blue / Horizon V450 / BV421 (448/45nm)
    2. Horizon V500 / BV510 / Alexa Fluor 430 (528/45nm)
    3. BV750 / BV786 / Qdot800 (755nmLP)
BD FACSMelody Cell Sorter - Basic Operation Procotol
Staff-in-charge:Ms. YAU, Ka Long (Erica)

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BD FACSAria SORP:

  • 488nm blue laser:
    1. FITC / GFP / Alexa Fluor 488 (530/30nm)
    2. PerCP-Cy5.5 / PerCP (695/40nm)
  • 640nm red laser
    1. APC (660/30nm)
    2. Alexa Fluor 700 (740/35nm)
    3. APC-Cy7 / APC-H7 (780/60nm)
  • 561nm yellow-green laser
    1. PE / DsRed (582/15nm)
    2. PE-Texas Red / mCherry / PI (610/20nm)
    3. PE-Cy5 (660/20nm)
    4. PE-Cy7 (780/60nm)
  • 405nm violet laser:
    1. BV421/Pacific Blue / Horizon V450 (450/50nm)
    2. BV510/AmCyan / Pacific Orange / Horizon V500 (525/50nm)
    3. BV605 / Qdot605 (610/20nm)
    4. BV650/ Qdot655 (660/20nm)
    5. BV711 (710/50nm)
    6. BV786 (780/60nm)
  • 355 nm UV laser:
    1. Hoechst blue / DAPI (450/50nm)
    2. Hoechst red (675/50)
    3. BUV395 (379/28nm)
FACSAria SORP - Basic Operation Procotol
Staff-in-charge:Dr. Liu, Jing (Emily)

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BD Influx:

  • 405nm Violet Laser:
    1. DAPI / Pacific Blue (425/26nm)
    2. Horizon V500 / Pacific Orange (520/35nm)
    3. QDot 605 (605/40nm)
  • 488nm Blue Laser:
    1. FITC (530/40nm)
    2. PerCP (670/30nm)
    3. PerCP-Cy5.5 (710/50nm)
  • 561nm Yellow-Green Laser:
    1. PE (585/29nm)
    2. mCherry / PE-CF594 (610/20nm)
    3. PE-Cy5 (670/30nm)
    4. PE-Cy5.5 (710/50nm)
    5. PE-Cy7 (750nmLP)
  • 640nm Red Laser:
    1. APC (670/30nm)
    2. APC-Alexa 700 (720/40nm)
    3. APC-H7 / APC-Cy7 (750nmLP)
    Kindly be noted that ONLY Regular or Experienced user of Aria I/SORP cell sorter who fulfills either one of the pre-requisites stated below is eligible for equipment usage due to the complicated operation of this cell sorting system:

    a. Having large sample size which takes at least 4 hours to complete on Aria I/SORP cell sorter.
    b. Neither Aria I nor Aria SORP can fulfill their experimental requirement.

    Training of BD InfluxTM Cell Sorting system will be held once per month based on first-come-first-serve principle. ONLY the training application of those who meet the prerequisite(s) stated above will be confirmed.

BD Influx Standard Operation Protocol - Basic Operation
Staff-in-charge:Ms. YAU, Ka Long (Erica)

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BD FACSCantoII Analyzer:

  • 488nm blue laser:
    1. FITC (530/30nm)
    2. PE / PI (585/42nm)
    3. PerCP / PerCP-Cy5.5 (670nmLP)
    4. PE-Cy7 (780/60nm)
  • 633nm HeNe laser:
    1. APC (660/20nm)
    2. APC-Cy7 (780/60nm)
FACSCantoII - Basic Operation Protocol
Staff-in-charge:Ms. YAU, Ka Long (Erica)

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Please click here for detailed comparison of two of our flow analyzers



BD LSR Fortessa Analyzer:

  • 405nm Violet Laser:
    1. BV421/Horizon V450 / Pacific Blue (450/50nm)
    2. BV510/Horizon V500 / Pacific Orange (525/50nm)
    3. BV605/QDot 605 (610/20nm)
    4. BV650 (660/20nm)
    5. BV711 (710/50nm)
    6. BV786 (780/60nm)
  • 488nm Blue Laser:
    1. BB515/ AF480/FITC (530/30nm)
    2. 7-AAD (660/20nm)
    3. BB700/ PerCP-Cy5.5 (710/50nm)
  • 561nm Yellow-Green Laser
    1. PE (586/15nm)
    2. PI / PE-Texas Red / mCherry (610/20nm)
    3. PE-Cy5 (670/30nm)
    4. PE-Cy5.5 (710/50nm)
    5. PE-Cy7 (780/60nm)
  • 640nm Red Laser
    1. APC (670/14nm)
    2. Alexa-Fluor 700 (730/45nm)
    3. APC-H7 / APC-Cy7 (780/60nm)
LSR Fortessa - Basic Operation Protocol (Tube Mode)
LSR Fortessa - Basic Operation Protocol (HTS Mode)
Staff-in-charge:Ms. YAU, Ka Long (Erica)

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ACEA NovoCyte Quanteon:

  • With 4 lasers, 25 colors, and 25 independent, highly sensitive detectors, the new NovoCyte Quanteon easily accommodates the large, multi-color panels found in today's highly progressive flow cytometry laboratories. Yet, with a number of key features such as auto compensation, as well as fixed and flexible signal collection options, the system is remarkably easy to set up for users of all levels and experience.
  • The NovoSampler Q, which can be integrated into different laboratory automation platforms, efficiently processes both FACS tubes (using a 40-tube rack) and 24-, 48-, 96-, and 384-well plates. The intuitive and industry leading NovoExpress software has been further advanced, providing an exceptional user experience in data acquisition, analysis and reporting.
  • Detailed optical configuration

  • ACEA NovoCyte Quanteon - Basic Operation Protocol
    Staff-in-charge:Dr. Liu, Jing (Emily)

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NovoCyte Advanteon BVR:

  • 405nm Violet Laser:
    1. Pacific Blue / DAPI / BV421 / Horizon V450 (445/45nm)
    2. BV510/ Horizon V500 / AmCyan / Alexa Fluor 430 (530/30nm)
    3. Pacific Orange (586/20nm)
    4. QDot605 / BV605 (615/20nm)
    5. QDot655 / BV650 (667/30nm)
    6. QDot705 (695/40nm)
    7. BV711 (710/50nm)
    8. Qdot800 / BV786 (780/60nm)
  • 488nm Blue Laser:
    1. BB515/ AF488 / FITC / GFP (530/30nm)
    2. PE (572/28nm)
    3. PE-Texas Red / PI (615/20nm)
    4. PerCP (667/30nm)
    5. PE-Cy5.5/ PerCP-Cy5.5 (695/40nm)
    6. PerCP-eFluor710 (725/40nm)
    7. PE-Cy7 (780/60nm)
  • 640nm Red Laser:
    1. APC (670/30nm)
    2. Alexa Fluor 680 (695/40nm)
    3. Alexa Fluor 700 (725/40nm)
    4. APC-H7 / APC-Cy7 (780/60nm)
Novocyte Advanteon BVR - Basic Operation Protocol
Staff-in-charge:Dr. Liu, Jing (Emily)

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NovoCyte Advanteon BVYG:

  • 405nm Violet Laser:
    1. Pacific Blue / DAPI / BV421 / Horizon V450 (445/45nm)
    2. BV510/ Horizon V500 / AmCyan / Alexa Fluor 430 (530/30nm)
    3. Pacific Orange (586/20nm)
    4. QDot605 / BV605 (615/20nm)
    5. QDot655 / BV650 (667/30nm)
    6. Qdot800 / BV786 (780/60nm)
  • 488nm Blue Laser:
    1. BB515/ AF488 / FITC / GFP (530/30nm)
    2. EYFP (586/20nm)
    3. PI (B) (615/20nm)
    4. PerCP (667/30nm)
    5. PE-Cy7 (B) (780/60nm)
  • 561nm Yellow-Green Laser:
    1. PE (586/20nm)
    2. PE-Texas Red / mCherry / PE-CF594 / PI (Y) (615/20nm)
    3. PE-Cy5 (667/30nm)
    4. PE-Cy7 (Y) (780/60nm)
Novocyte Advanteon BVYG - Basic Operation Protocol
Staff-in-charge:Dr. Liu, Jing (Emily)

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