EY1920 Year Book

125 124 Leung Yee Nok Clemence Duties • Accompanying clients to clinic or hospital visits and facilitating communication between clients and healthcare professionals • Arranging family planning visits for clients • Assisting staff at PathFinders Health Centre • Taking part in case manager meetings and operations meetings • Creating educational leaflets for clients • Giving health advice under staff supervision to clients • Assisting case managers in their duties e.g. translation in client meetings, buying supplies • Clerical work and data entry Academic output Internal report on clients’ contraceptive experience at the Family Planning Association of Hong Kong and PathFinders Personal takeaways Although we will meet patients from all walks of life in the future, there are still people in need who are barred from healthcare due to their immigration status. Clients I met had to face their own pregnancy without much support, which added many uncertainties to their lives in Hong Kong, and some were even vulnerable to various dangers, such as domestic violence or drug abuse. PathFinders’ work made me realise the importance of community networks in supporting clients who fall through the cracks of society and education in empowering them to avoid recurrent involvement in difficult situations. Enrichment Year has exposed me to new experiences, most of which have brought me joy and unforgettable memories. I have also had the chance to reflect on myself, which helped me organise my thoughts and hopefully would prepare me for a fresh start next year. Service targets Migrant mothers (mainly current and former foreign domestic workers) and their children MBBS Enrichment Year 2019/20 Leung Yee Nok Clemence Asia: Past, Present, Future 1 Asian history, starting from continent formation, development of societies, religions and ideologies to future global projections in the Big History timeline Disease in Ageing Holistic management of health of the elderly and common diseases emerging with old age Language and Social Context Relationship between social differences e.g. gender, social class and development of change and varieties in language Psychology 1002 Learning and motivation, perception, psychological abnormalities, cognitive processes, mental abilities and neuroscience Other experiences • Moving into a flat and cultivating friendships with classmates and five flatmates • Travelling to Melbourne, Tasmania and Adelaide • Learning about Australian history and appreciating nature Sem 1 < IC - HKUWW Exchange Programme at T he University of Sydney, Australia >; Sem 2 < SH - Internshi p at Path F inders, Hong Kong > Characterisation of mycoviruses in Aspergillus spp. Introduction Key questions Methods and materials Screening for positive Aspergillus strains Molecular cloning and sequencing Results dsRNA segments in Aspergillus spp. Nucleotide sequence • Are mycoviruses present in Aspergillus spp. isolated from local clinical samples? • If mycoviruses are present, what are their prevalence and genomic organisation? • How the prevalence and genomic organisation of these mycoviruses differ from studies conducted in other regions/countries? • Aspergillus is a medically important fungal species that commonly causes pulmonary aspergillosis. With the rise of azole resistance by Aspergillus , alternative therapeutic measures should be worked out. • Mycoviruses are viruses that infect fungi specifically. They commonly possess double-stranded (ds) RNA genome. Mycoviruses that can cause hypovirulence or a killer phenotype on their hosts can be used as an alternative agent to control fungal infection. • 3 positive strains (lane 1-3) were observed out of 59 strains. • 2 distinct bands were observed from 2 strains (lane 1-2). Sizes of the bands were consistent with the members in the family Partitiviridae . • 4 distinct bands were observed from 1 strain (lane 3). Sizes of the bands were consistent with the members in the family Polymycoviridae . • Positive clones were identified from LB agar, which were used for the extraction of plasmids. • Linearised plasmids with size ~ 3.5 kbp were observed in gel photos. Those plasmids with the targeted insert were selected, which were further analysed by sequencing. • Sequencing results will be analysed in the future, which can be used to identify the family of the discovered mycoviruses, genomic organisation and overall base composition. Fungus culture and spore collection RNA extraction and gel electrophoresis to screen for positive strains (infected with mycovirus) Reverse transcription (RT) and polymerase chain reaction (PCR) performed on RNA extracted from positive strains Cloning of PCR products into a vector to form a ligated plasmid to transform E. coli cells, which are cultured on Luria Bertani (LB) agar Isolation of positive clones for plasmid preparation for subsequent PCR and sequencing MBBS Enrichment Year 2019/20 Li Cheuk Yin Boris Full Year < RA - Sc reening of mycov iru s e s in Asp ergillu s spp at D e p art m ent of Mi c r ob i o l o g y, HK U, H o ng K o ng > Li Cheuk Yin Boris