Faculty Core Facility
Address: L6-11, 6/F, Laboratory Block, 21 Sassoon Road, Pokfulam Hong Kong

Tel: 39177557
Fax: 29864297
E-mail: corefac@hku.hk

Equipment — Flow Cytometry

BD FACSAria I Cell Sorter:

FACS FACSAria I - Basic Operation Protocol

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BD FACSAria SORP:

  • 488nm blue laser:
    1. FITC / GFP / Alexa Fluor 488 (530/30nm)
    2. PerCP-Cy5.5 / PerCP (695/40nm)
  • 640nm red laser
    1. APC (660/20nm)
    2. APC-Cy7 / APC-H7 (780/60nm)
  • 561nm yellow-green laser
    1. PE / DsRed (582/15nm)
    2. PE-Texas Red / mCherry / PI (610/20nm)
    3. PE-Cy5 (660/20nm)
    4. PE-Cy7 (780/60nm)
  • 405nm violet laser:
    1. Pacific Blue / Horizon V450 (450/50nm)
    2. AmCyan / Pacific Orange / Horizon V500 (525/50nm)
  • 355 nm UV laser:
    1. Hoechst blue / DAPI (450/50nm)
    2. Hoechst red (675/50)
FACSAria SORP - Basic Operation Procotol

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BD Influx:

  • 405nm Violet Laser:
    1. DAPI / Pacific Blue (425/26nm)
    2. Horizon V500 / Pacific Orange (520/35nm)
    3. QDot 605 (605/40nm)
  • 488nm Blue Laser:
    1. FITC (530/40nm)
    2. PerCP (670/30nm)
    3. PerCP-Cy5.5 (710/50nm)
  • 561nm Yellow-Green Laser:
    1. PE (585/29nm)
    2. mCherry / PE-CF594 (610/20nm)
    3. PE-Cy5 (670/30nm)
    4. PE-Cy5.5 (710/50nm)
    5. PE-Cy7 (750nmLP)
  • 640nm Red Laser:
    1. APC (670/30nm)
    2. APC-Alexa 700 (720/40nm)
    3. APC-H7 / APC-Cy7 (750nmLP)
    Kindly be noted that ONLY Regular or Experienced user of Aria I/SORP cell sorter who fulfills either one of the pre-requisites stated below is eligible for equipment usage due to the complicated operation of this cell sorting system:

    a. Having large sample size which takes at least 4 hours to complete on Aria I/SORP cell sorter.
    b. Neither Aria I nor Aria SORP can fulfill their experimental requirement.

    Training of BD InfluxTM Cell Sorting system will be held once per month based on first-come-first-serve principle. ONLY the training application of those who meet the prerequisite(s) stated above will be confirmed.


BD FACSCantoII Analyzer:

  • 488nm blue laser:
    1. FITC (530/30nm)
    2. PE / PI (585/42nm)
    3. PerCP / PerCP-Cy5.5 (670nmLP)
    4. PE-Cy7 (780/60nm)
  • 633nm HeNe laser:
    1. APC (660/20nm)
    2. APC-Cy7 (780/60nm)
FACSCantoII - Basic Operation Protocol

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Please click here for detailed comparison of two of our flow analyzers



BD LSR Fortessa Analyzer:

  • 405nm Violet Laser:
    1. Horizon V450 / Pacific Blue (450/50nm)
    2. Horizon V500 / Pacific Orange (525/50nm)
    3. QDot 605 (610/20nm)
  • 488nm Blue Laser:
    1. FITC (530/30nm)
    2. PerCP-Cy5.5 (710/50nm)
  • 561nm Yellow-Green Laser
    1. PE (586/15nm)
    2. PI / PE-Texas Red / mCherry (610/20nm)
    3. PE-Cy5 (670/30nm)
    4. PE-Cy5.5 (710/50nm)
    5. PE-Cy7 (780/60nm)
  • 640nm Red Laser
    1. APC (670/14nm)
    2. Alexa-Fluor 700 (730/45nm)
    3. APC-H7 / APC-Cy7 (780/60nm)
LSR Fortessa - Basic Operation Protocol (Tube Mode)
LSR Fortessa - Basic Operation Protocol (HTS Mode)

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